BEGIN:VCALENDAR VERSION:2.0 PRODID:-//132.216.98.100//NONSGML kigkonsult.se iCalcreator 2.20.4// BEGIN:VEVENT UID:20250929T204055EDT-8875xdl0Vz@132.216.98.100 DTSTAMP:20250930T004055Z DESCRIPTION:DNA is a reliable and programmable nanofabrication building blo ck. Taking advantage of its nanoscale features\, I used it extensively to generate complex reconfigurable assemblies with many recent potential appl ications. My PhD research can be divided into four main parts: (i) synthes is optimization (Hariri et. al. 2015)\, (ii) structural characterization ( Hariri et. al. 2017)\, (iii) monitoring dynamic structures (Rahbani et al. 2015)\, and (iv) studying structural dynamics (Platnich et. al. 2017). Fi rst\, a solid-phase synthesis strategy and its visualization through SMF s pectroscopy was devised to assemble nanotubes in a stepwise fashion\, with a full control over their size and sequence pattern. This method paves th e way for the production of custom-made DNA nanotubes with fewer structura l flaws than the spontaneous-assembly method. Second\, SMF photobleaching and two-color approaches were combined to provide a systematic way of asse ssing the polydispersity\, stoichiometry and degree of defectiveness of DN A nanotubes. Third\, in situ SMF was employed to introduce structural chan ges into DNA nanotubes by dynamically adjusting one or several of the edge lengths between the building blocks using strand displacement and loops. This is interesting for sensing applications\, especially when the analyte produces large scale\, detectable structural changes. Lastly\, dynamics o f DNA nanotubes\, reconfigured in response to site-specific deletion of DN A strands\, were investigated using SMF microscopy. This strategy enables to develop a better understanding of the collective structural changes wit hin DNA structures. Together\, the different methods developed underline t he importance of SMF techniques as powerful tools which can advance the fi eld of DNA nanotechnology by enabling the production of well-defined high- quality objects that can meet the designer’s compositional and dynamic spe cifications.\n\nMy current postdoctoral work focuses on developing real-ti me DNA-based biosensors: The capacity to measure specific biomolecules rap idly in vivo would provide clinicians with a valuable window into patients ’ health and their response to therapeutics. The Soh lab developed the fir st “universal real-time biosensor technology” capable of continuously trac king a wide range of circulating molecules in living animals (Mage et. al. 2017). This real-time biosensor requires no exogenous reagents\, operates at room temperature\, and can be reconfigured to measure a wide range of target molecules by exchanging probes in a modular manner. At the heart of the sensor is an aptamer probe\, which is labeled with a redox reporter a nd immobilized onto an electrode within a microfabricated device. These ap tamer probes fold reversibly\, enabling continuous tracking of rising and falling concentrations of the biomarker in real-time. Importantly\, the ap tamer probe governs the sensor’s sensitivity\, specificity and temporal re solution. The Soh Lab has achieved many milestones in the development of a dvanced techniques that are now in widespread use for aptamer discovery\, including the click particle display (click-PD) method which allows for th e high-throughput screening and isolation of chemically modified\, non-nat ural aptamers (Wang et. al. 2016).\n DTSTART:20170123T180000Z DTEND:20170123T193000Z LOCATION:OM 10\, Maass Chemistry Building\, CA\, QC\, Montreal\, H3A 0B8\, 801 rue Sherbrooke Ouest SUMMARY:Chemical Society Seminar: Dr. Amani Hariri - Advancing DNA Nanotech nology Using Single Molecule Fluorescence Methodologies URL:/chemistry/channels/event/chemical-society-seminar -dr-amani-hariri-advancing-dna-nanotechnology-using-single-molecule-283507 END:VEVENT END:VCALENDAR